The role of human papillomavirus DNAs in cervical carcinoma and risk of lymph node metastasis

Abstract Background: Tumor microenvironment (TME) plays a particularly important role in the progression, invasion and metastasis of cervical carcinoma (CC). Tumor-associated macrophages (TAMs) are significant components of cervical cancer microenvironment. However, the result of studies on the correlation between TAMs and progression in CC is still controversial. This research is aimed at investigating the relationship between TAMs and progression in CC.Method: A total of 100 patients with CC were included in this study. The correlation between TAMs and clinicopathologic features was studied. Also, a systematic literature search was conducted from legitimate electronic databases. This is the first meta-analysis to specifically evaluate the role of different types of TAMs in TME of cervical cancer.Results: In the meta-analysis, high stromal CD68+ TAMs density was relevant to lymph node metastasis (WMD = 11.89, 95% CI 5.30 to 18.47). At the same time, CD163+ M2 TAM density was associated with lymph node metastasis (OR = 2.42, 95% CI 1.09 to 5.37; WMD = 39.37, 95% CI 28.25 to 50.49) and International Federation of Obstetrics and Gynecology (FIGO) stage (WMD = -33.60, 95% CI -45.04 to -22.16). This was confirmed in 100 experimental studies of CC. It supported a critical role of TAMs as a prospective predictor for cervical cancer.Conclusion: Taken together, CD68+ TAM and CD163+ M2 TAM infiltration in cervical cancer was association with tumor progression. And CD163+ M2 TAM infiltration was associated with more advanced FIGO stage and lymph node metastasis in CC.

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Unclear role of human papillomavirus infection in response to adjuvant radiotherapy: conflicting results on the prognostic significance of irradiation in vulvar cancer patients with lymph node metastasis

Current Medical Research and Opinion ◽

10.1080/03007995.2016.1174842 ◽

2016 ◽

Vol 32 (8) ◽

pp. 1387-1388

Author(s):

Jacek J. Sznurkowski

Keyword(s):

Human Papillomavirus ◽

Lymph Node ◽

Lymph Node Metastasis ◽

Adjuvant Radiotherapy ◽

Vulvar Cancer ◽

Prognostic Significance ◽

Node Metastasis ◽

Human Papillomavirus Infection ◽

Papillomavirus Infection

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The correlation between tumor-associated macrophage infiltration and progression in cervical carcinoma

Bioscience Reports ◽

10.1042/bsr20203145 ◽

2021 ◽

Author(s):

Fan Guo ◽

Wei na Kong ◽

Gang Zhao ◽

Zhen zhen Cheng ◽

Le Ai ◽

...

Keyword(s):

Cervical Cancer ◽

Lymph Node ◽

Tumor Microenvironment ◽

Lymph Node Metastasis ◽

Cervical Carcinoma ◽

Meta Analysis ◽

Critical Role ◽

Figo Stage ◽

Node Metastasis

Tumor microenvironment (TME) plays a particularly important role in the progression, invasion and metastasis of cervical carcinoma (CC). Tumor-associated macrophages (TAMs) are significant components of the tumor microenvironment in CC. However, the results of studies on the correlation between TAMs and progression in CC are still controversial. This research aimed to investigate the relationship between TAMs infiltration and progression in CC. A total of 100 patients with CC were included in the study. The correlation between TAMs and clinicopathologic features was studied. Besides, a systematic literature search was conducted from legitimate electronic databases to specifically evaluate the role of TAMs in TME of cervical carcinoma. In the meta-analysis, high stromal CD68+ TAMs density was relevant to lymph node metastasis (WMD = 11.89, 95% CI 5.30 to 18.47). At the same time, CD163+ M2 TAM density was associated with lymph node metastasis (OR = 2.42, 95% CI 1.09 to 5.37; WMD = 39.37, 95% CI 28.25 to 50.49) and FIGO stage (WMD = -33.60, 95% CI -45.04 to -22.16). This was further confirmed in the experimental study of 100 tissues of cervical cancer. It supported a critical role of TAMs as a prospective predictor of cervical cancer. In conclusion, CD68+ TAM and CD163+ M2 TAM infiltration in CC were associated with tumor progression. And CD163+ M2 TAM infiltration was associated with more advanced FIGO stage and lymph node metastasis in CC.

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Abstract PS1-54: Risk factors for lymph node metastasis and the role of SLNB in microinvasive breast cancer

10.1158/1538-7445.sabcs20-ps1-54 ◽

2021 ◽

Author(s):

Pill Sun Paik ◽

Min Kyung Cho ◽

Juneyoung Ahn ◽

Chang Ik Yoon ◽

Tae-Kyung Yoo ◽

...

Keyword(s):

Breast Cancer ◽

Risk Factors ◽

Lymph Node ◽

Lymph Node Metastasis ◽

Node Metastasis ◽

Microinvasive Breast Cancer

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The role of microvessel density, lymph node metastasis, and tumor size as prognostic factors of distant metastasis in colorectal cancer

Oncology Letters ◽

10.3892/ol.2017.5959 ◽

2017 ◽

Vol 13 (6) ◽

pp. 4327-4333 ◽

Cited By ~ 8

Author(s):

Tomonari Cho ◽

Eisuke Shiozawa ◽

Fumihiko Urushibara ◽

Nana Arai ◽

Toshitaka Funaki ◽

...

Keyword(s):

Colorectal Cancer ◽

Prognostic Factors ◽

Lymph Node ◽

Lymph Node Metastasis ◽

Distant Metastasis ◽

Microvessel Density ◽

Tumor Size ◽

Node Metastasis

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FGFR3 promotes the growth and malignancy of melanoma by influencing EMT and the phosphorylation of ERK, AKT, and EGFR

BMC Cancer ◽

10.1186/s12885-019-6161-8 ◽

2019 ◽

Vol 19 (1) ◽

Cited By ~ 6

Author(s):

Lei Li ◽

Shuai Zhang ◽

Hao Li ◽

Haiyan Chou

Keyword(s):

Cell Proliferation ◽

Lymph Node ◽

Lymph Node Metastasis ◽

Colony Formation ◽

Caspase 3 ◽

Growth Factor Receptor ◽

Invasion And Migration ◽

Node Metastasis ◽

And Migration

Abstract Background Overexpression of fibroblast growth factor receptor 3 (FGFR3) has been linked to tumor progression in many types of cancer. The role of FGFR3 in melanoma remains unclear. In this study, we aimed to uncover the role of FGFR3 in the growth and metastasis of melanoma. Methods FGFR3 knockdown and overexpression strategies were employed to investigate the effects of FGFR3 on colony formation, cell apoptosis, proliferation, migration, and in vitro invasion, along with the growth and metastasis of melanoma in a xenografts mouse model. The protein expression levels of extracellular signal-regulated kinase (ERK), protein kinase B (AKT), epidermal growth factor receptor (EGFR), and epithelial-mesenchymal transition (EMT) markers were determined by Western blot analysis. Results The mRNA expression of FGFR3 was higher in melanoma tissues than normal healthy tissues. FGFR3 expression in cutaneous malignant melanoma (CMM) tissues was positively correlated with the Breslow thickness and lymph node metastasis. In A357 cells, knockdown of the FGFR3 gene decreased the colony formation ability, cell proliferation, invasion, and migration, but increased the caspase 3 activity and the apoptosis rate; overexpression of FGFR3 increased the colony formation ability, cell proliferation, invasion, and migration, but decreased the caspase 3 activity and apoptosis rates. FGFR3 knockdown also upregulated E-cadherin, downregulated N-cadherin and vimentin, and decreased the phosphorylation levels of ERK, AKT, and EGFR. In the MCC xenografts mice, knockdown of FGFR3 decreased tumor growth and metastasis. Conclusions FGFR3, which is highly expressed in CMM tissues, is correlated with increased Breslow thickness and lymph node metastasis. FGFR3 promotes melanoma growth, metastasis, and EMT behaviors, likely by affecting the phosphorylation levels of ERK, AKT, and EGFR.

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